Description
ABSTRACT
The bacterial isolate from the teeth was determined by using standard microbiological method. About 0.1ml Aliguot of the 10-2 dilution was aseptically sampled in bacteriological media. The result obtained shows that the bacteriological level detected in the various teeth ranged from 40.0 x10-1, 60.0 x10-1,41.0×10-1, 18.0 X 10-1, 20.0 X 10-1, 15.0 x 10-1, 20.0 x 10-1. The organism isolated were gram positive rod and gram positive cocci. The antimicrobial activity of chewing stick was performed from isolates gotten from the teeth. The organisms included are gram positive cocci and gram positive rod, decoction method was used for the extraction of the active compounds from the plant. The result revealed that the chewing stick showed activity against gram positive rod at the concentration of 62.5mg/ml with the zone of inhibition of 7mm and 5mm. The minimum inhibitory concentration (MIC mg/ml) and minimum bactericidal concentration (MBC mg/m) was determined to show that the extract exhibited the growth of the test organism with the MIC between 62.5mg/ml.
TABLE OF CONTENT
Title page
Certification
Dedication
Acknowledgment
Abstract
Table of content
CHAPTER ONE
1.0 Introduction
CHAPTER TWO
2.0 Literature Review
2.1 Plants commonly used as chewing sticks
2.2 Mechanism of Action
2.3 Effect of Dental Plaque
2.4 Effect on Periodontal health
2.5 Antibacterial Activity
2.6 Cytotoxic Effects
CHAPTER THREE
3.0 Materials and Methods
3.1 Collection of plant Material
3.2 Methodology
3.3 Extraction Procedures of Chewing Stick
3.4 Preparation of Medium
3.4.1 Preparation of Nutrient Agar
3.4.2 Preparation of Muller Hilton Agar
3.5 Sterilization of Media and Glasses
3.5.2 Preparation of paper Disc
3.6 Determination of Zones of Inhibition
3.6.1 Minimum Inhibitor Concentration Tests (MIC)
3.7 Minimum Bactericidal Concentration Test (MBC)
3.8 Grams staining techniques
CHAPTER FOUR
4.0 Result and discussion
4.1 Discussion
CHAPTER FIVE
5.0 Conclusion and Recommendation
5.1 Conclusion
5.2 Recommendations
References